Alteration of Serum Bilirubin and Myeloperoxidase Concentration Associated Moderate Intensity Exercise Among Novice Students Athletes

Bilirubin and Myeloperoxidase (MPO) are both biochemical parameters that play a vital role in maintaining various physiological activities of the human body. Objective: To scrutinize the Alteration of serum bilirubin and MPO Concentration Associated with moderate-intensity exercise among Novice student-athletes. Methods: The study participants involved twenty (20) newly registered athletes of Punjab University and were placed randomly in a control group (CG, N=10) and an experimental group (EG, N=10). Five (05) ml blood samples were collected from each subject, and thus a different identi�cation code was given to each blood sample. Bilirubin and MPO test were conducted in the home care laboratory. A self-made moderate-intensity exercise intervention of eight (08) weeks was applied to EG. Pre and post-test data were administered through a statistical package for social sciences (SPSS, version-26.0), and thus mean, standard deviation, and T-score were used as statistical tools for analysis. Results: Results revealed that there is a signi�cant difference in pre and post-test of EG in terms of MPO (0.049 < 0.05), Likewise in terms of Bilirubin of CG, there is a signi�cant difference in pre and post-test results (.546> 0.05). Conclusions: Similarly, based on analysis, the researcher concluded that moderate Intensity Exercise caused a signi�cant alteration in serum bilirubin and MPO among Novice student-athletes.


M E T H O D S
system which helps in avoiding oxidative stress and maintaining a blood redox state.As a result of all the above critical analyses of previous studies, it is clear that exercise has a signi cant impact on antioxidants such as Bilirubin and MPO.Is there any effect of moderate intensity on Bilirubin and MPO?To discover this fact, the researcher intends to conduct a study titled Alteration of Serum Bilirubin and MPO Concentration Associated Moderate Intensity Exercise among Novice Students Athletes.
As the study was associated with the alteration of MPO concentration with Moderate-intensity exercise among novice student-athletes therefore an experimental research approach was used with quantitative research methods.The study participants comprised twenty (20) newly admitted athletes of Punjab University.Based on Cohen's suggestion, the sample size was measured by using the statistical test G* Power, providing the size effect of the sample size.i.e. small, median, and large, and thus participants of the study were randomly placed into two groups i.e. control group (CG, N=10) and an experimental group (EG, N=10).In addition, the criteria for selecting the subjects were; subjects aged not under 18 and more than 25 years, subjects using no meditations as well as nutritional supplementations, subjects who voluntarily participated in the study, and subjects having no chronic health problems.Written informed consent was taken from all topics, and thus ethical approval was taken from the Department of Sports Sciences & Physical Education, University of the Punjab, Lahore, Pakistan (Ref.SPSS/28/10 2023).Five (05) ml blood samples were collected from each subject, and thus a different identi cation code was given to each blood sample.Bilirubin and (MPO) tests were conducted in the home care laboratory.A self-made moderate-intensity exercise intervention of eight (08) weeks was applied to EG. Application of exercise on the EG was started from 1st Nov to 29 December 2023 which was personally monitored by the researcher.Pre and post-test data were processed through a statistical package for social sciences (SPSS, version-26), and thus appropriate statistical tools i.e. mean, standard deviation, and t-test were used for analysis.

R E S U L T S
Table 1 expressed Anthropometric measurements as a whole in Age, Weight, Height, and BMI of both CG and EG.The M and SD in terms of age was 20.22 ± 1.44.The M and SD in terms of height was 174.44 ± 6.76, the M and SD in terms of weight (pre) was 68.33 ± 9.01, M and SD in terms of weight (post) was 62.51 ± 8.28.[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] Results revealed that there is a signi cant difference in pre and post-test of EG in terms of MPO (0.049 < 0.05), Likewise in terms of Bilirubin of CG, there is a signi cant difference in pre and post-test results (.546 > 0.05).In line with these emerging ndings, the study conducted expressed that moderate-intensity exercise signi cantly increases the level of serum Bilirubin level among subjects [10].The same results were also drawn by that exercise with different volumes and intensity particularly high-intensity exercise (HIT) increases the Bilirubin level among adults [13,14].
Likewise, the study conducted by Witek et al.,  Based on data analysis and ndings, the researcher concluded that moderate-intensity exercise caused a signi cant alteration in serum bilirubin and MPO.Considering this conclusion, the researcher recommended that regular exercise, particularly moderate-intensity exercise, may be performed due to its close linkage with serum bilirubin MPO.

Table 1 :
Anthropometric measurements as a whole in Age, Weight, Height and BMI of both CG and EG p value=.005N=10Table3indicates the pre and post-test results of CG and EG in terms of MPO.A statistically signi cant difference was found in the pre and post-test of EG in terms of MPO (0.000< 0.05) On the other hand, no statistical difference was found in the pre and post-test of CG in terms of MPO (.546 > 0.05).

Table 2 :
Mean difference between CG and EG in term of Bilirubin before and after exercise

Table 3 :
Illustrate the differences between Pre and Post of CG and EG in term of MPO before and after exercise found that according to the normal range, athletes participating in aggressive sports can achieve higher levels of total blood level[15].The result of the current study shows that there is a signi cant difference in the pre and post-test of EG in terms of MPO (0.000 < 0.05) On the other hand, no statistical difference was found in the pre and post-test of CG in terms of MPO (.546 > 0.05).Likewise, Belcastro et al., shows that MPO activity is raised in most rat tissues and not solely in skeletal muscle[16].Such an emerging concept was supported that aerobic exercise helps in managing or controlling different health complications such as high blood pressure, heart problems, and so on[17][18][19][20].Intensive aerobic training with vitamin C may have healthier e cacy.The same result was drawn that exercise modalities have a signi cant impact on MPO among the subjects of EG as compared to CG[21, 22].